High-throughput measurement of log d and pka
WebFeb 15, 2003 · A procedure is described for measuring pKa values in a short time, e.g., 4 min/assay. Samples, as 10 mM solutions, are prepared in DMSO in 96-well plates. ... High-throughput measurement of pKa values in a mixed-buffer linear pH gradient system Anal Chem. 2003 Feb 15;75(4):883-92. doi: 10.1021/ac020329y. WebItraconazole is a base with a pKa of 3.7, and log P greater than 5. At high pH it is not ionized and is almost insoluble. It ion-izes at low pH, and is rather more soluble at pH 5 than at pH 6. ... pKa values. High-Throughput Measurement of Drug pKa Values for ADME Screening John Comer, Karl Box* Keywords: ionization constant, drug absorption, ADME
High-throughput measurement of log d and pka
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WebApr 11, 2024 · Visualization-based techniques are a useful means to monitor and analyze drugs in the brain, for example, an isotopically labeled drug makes it possible to measure its concentrations in the brain with the assistance of a PET or SPECT scanner, and the mass spectrometry imaging (MSI) technique enables the compound-specific imaging of drug … WebNov 5, 2003 · A high-throughput pK a screening method based on pressure-assisted capillary electrophoresis (CE) and mass spectrometry (MS) is presented. Effects of buffer …
WebThis study describes a 96-well reverse phase HPLC method, simultaneously determining LogD, LogP, and pK(a) values of drugs in a throughput mode. The LogD and LogP values of each compound were determined, based on the octanol-aqueous partitioning behavior of the charged and non-charged species under different pH values. WebHigh‐Throughput Measurement of log D and pKa. Electrodes are now used routinely in many research laboratories and in an increasing number of industrial quality control …
WebApr 10, 2024 · Subsequent high-throughput single-cell sequencing enables integrated analysis of the single-cell transcriptome, secretion and phenotypes (Fig. 1e, f). TRAPS-seq enables multimodal single-cell ... WebDec 22, 2006 · A high-throughput method has been developed for lipophilicity measurement. ... The composition is 2:1 (w/w) dioctyl sebacate and PVC. With six repeats, log P pw values of 15 solutes have been determined in one 96-well microplate in 4 h. A linear relationship between log ... High‐Throughput Measurement of Physicochemical …
WebJun 1, 2003 · Two high-throughput assays, Calcein-AM and ATPase, identify Pgp inhibitors and modulators, which may or may not be transport substrates. A pharmacophore model …
WebThe medium-throughput miniaturized protocols described herein, for determination of log P, log D and pKa, are straightforward to set up and require very small quantities of sample ( … cipriani sherry netherlandWebIn conclusion, we validate IFC as a viable high throughput tool to detect and measure PEG-mediated mitochondrial fusion activity in mammalian cells. This technique will become invaluable as we seek to unravel the intricacies of mitochondrial dynamics and to understand how the interplay of fusion and fission are regulated in a variety of human ... ciprianis grand centralWebSolubility is a property of central importance for the use of proteins in research in molecular and cell biology and in applications in biotechnology and medicine. Since experimental methods for measuring protein solubility are material intensive and dialysis long sleeve shirts with zipperWebThe measurement of capacitance shows that higher frequencies are most suited for cell spreading because they increase cell coverage of the electrode. At high frequency ranges, measurement is more sensitive to changes in cell attachment and spreading at the electrode. However, 10–40 kHz is the main frequency range used for measuring … ciprianis on 63rdWebJan 12, 2024 · A faster method of log P determination makes use of high-performance liquid chromatography. ... Log D is thus pH dependent, hence the one must specify the pH at which the log D was measured. Of particular interest is the log D at pH = 7.4 (the physiological pH of blood serum). ... For compounds with a pKa close to physiological pH it may be ... cipriani school belmontWebdownload file Automated HT pKa and logD Measurements for SAMPL8.pdf 1 MB [opens in a new tab] cloud_download pdf : 1 MB We are encoding your file so that it is compatible … cipriani softwareWebApr 12, 2024 · A major shortcoming in the implemented protocol was the time consumed prior to SERS measurements, which impaired high-throughput assays: on one hand, preparing the SERS substrates by drop casting takes time (>1 h); on the other hand, the supernatants to be spiked onto the SERS substrates had to be collected by hand. dialysis low blood pressure